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pe anti ulbp4  (R&D Systems)


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    R&D Systems pe anti ulbp4
    Pe Anti Ulbp4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+ulbp4/Human+ULBP-4%2FRAET1E+PE-conjugated+Antibody/pmc11706306-119-107-109
    Average 93 stars, based on 3 article reviews
    pe anti ulbp4 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Control:

    Article Title: NKG2D signaling between human NK cells enhances TACE-mediated TNF-α release
    Article Snippet: PE anti-NKG2D (1D11), PE-Cy7 anti-CD16 (B73.1), BV650 anti-CD62L (DREG-56) and PE Mouse IgG1 Isotype control (MOPC-21) were purchased from BioLegend. .. PE anti-MICA/B (159207), PE anti-ULBP1 (170818), PE anti-ULBP2/5/6 (165903), PE anti-ULBP3 (166510), PE anti-ULBP4 (709116), PE anti-TACE (FAB9301P), PE Mouse IgG 2A Isotype Control (20102), PE Mouse IgG 2B Isotype Control (133303), purified anti-NKG2D (149810) and Mouse IgG1 Isotype control (11711) were purchased from R&D Systems. .. Anti-TACE (D1(A12)) was purchased from EMD Millipore.

    Purification:

    Article Title: NKG2D signaling between human NK cells enhances TACE-mediated TNF-α release
    Article Snippet: PE anti-NKG2D (1D11), PE-Cy7 anti-CD16 (B73.1), BV650 anti-CD62L (DREG-56) and PE Mouse IgG1 Isotype control (MOPC-21) were purchased from BioLegend. .. PE anti-MICA/B (159207), PE anti-ULBP1 (170818), PE anti-ULBP2/5/6 (165903), PE anti-ULBP3 (166510), PE anti-ULBP4 (709116), PE anti-TACE (FAB9301P), PE Mouse IgG 2A Isotype Control (20102), PE Mouse IgG 2B Isotype Control (133303), purified anti-NKG2D (149810) and Mouse IgG1 Isotype control (11711) were purchased from R&D Systems. .. Anti-TACE (D1(A12)) was purchased from EMD Millipore.

    Staining:

    Article Title: NK Receptor Signaling Lowers TCR Activation Threshold, Enhancing Selective Recognition of Cancer Cells by TAA-Specific CTLs
    Article Snippet: .. Antibodies used for surface staining are as follows: APC anti-NKG2D (BD Biosciences Cat# 558071, RRID:AB_398654), BV510 anti-NKG2D (BioLegend Cat# 320815, RRID:AB_2562746), FITC anti-DNAM-1 (BD Biosciences Cat# 559788, RRID:AB_397329), BV510 anti-DNAM-1 (BioLegend Cat# 338330, RRID:AB_2728300), BV786 anti-CD8 (BD Biosciences Cat# 563823, RRID:AB_2687487), PerCP/Cyanine5.5 anti-TIGIT (BioLegend Cat# 372717, RRID:AB_2632932), APC anti-CD112R (BioLegend Cat# 301505, RRID:AB_2876586), BV421 anti-CD96 (BioLegend Cat# 338417, RRID:AB_2629536), BV785 anti-PD-1 (BioLegend Cat# 329929, RRID:AB_11218984), BV605 anti-TIM-3 (BioLegend Cat# 345018, RRID:AB_2563859), PE anti-PVR (BioLegend Cat# 337610, RRID:AB_2174019), PE anti-Nectin-2 (BioLegend Cat# 337410, RRID:AB_2269088), PE anti-MICA/MICB (BioLegend Cat# 320906, RRID:AB_493193), PE anti-ULBP1 (R and D Systems Cat# FAB1380P, RRID:AB_2687471), PE anti-ULBP3 (R and D Systems Cat# FAB1517P, RRID:AB_10719122), PE anti-ULBP4 (R and D Systems Cat# FAB6285P, RRID:AB_3083729), and PE anti-ULBP2/5/6 (R and D Systems Cat# FAB1298P, RRID:AB_2214693). ..



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    a. Flow cytometry gating strategy on PDTO cells for analysis of surface staining. Selected cells were gated on single, live cells before quantification of staining signal. b. Histogram representation and count for surface staining of MHC-I, PD-L1, and β2m expression on two PDTO lines B2MWT and B2MKO after IFNγ pre-stimulation. Staining with isotype antibodies for each fluorochrome (PE, APC and FITC) were included as negative control. c. Flow cytometry gating strategy on γδ T cell samples for analysis of intracellular staining to test antitumor reactivity upon PDTO stimulation. Lymphocyte population was further gated on single cells, live and CD3+ cells, γδ TCR+ cells and CD8+ as well as CD8–CD4– cells. Reactivity of the sample was based on IFNγ+ cells of the selected population. d. Histogram representation and count for surface staining of NKG2D ligands MICA/B, ULBP1, ULBP2/5/6, ULBP3, and <t>ULBP4</t> on two PDTO lines B2MWT and B2MKO after IFNγ pre-stimulation. e. Flow cytometry gating strategy on γδ T cell samples for analysis of intracellular staining after stimulation with PDTOs in the presence of NKG2D ligand blocking. Lymphocyte population was further gated on single cells, live and CD3+ cells, followed by γδ TCR+ and CD8+ as well as CD8– cells. Reactivity of final population was based on IFNγ+ or CD107a+ cells.
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    Image Search Results


    a. Flow cytometry gating strategy on PDTO cells for analysis of surface staining. Selected cells were gated on single, live cells before quantification of staining signal. b. Histogram representation and count for surface staining of MHC-I, PD-L1, and β2m expression on two PDTO lines B2MWT and B2MKO after IFNγ pre-stimulation. Staining with isotype antibodies for each fluorochrome (PE, APC and FITC) were included as negative control. c. Flow cytometry gating strategy on γδ T cell samples for analysis of intracellular staining to test antitumor reactivity upon PDTO stimulation. Lymphocyte population was further gated on single cells, live and CD3+ cells, γδ TCR+ cells and CD8+ as well as CD8–CD4– cells. Reactivity of the sample was based on IFNγ+ cells of the selected population. d. Histogram representation and count for surface staining of NKG2D ligands MICA/B, ULBP1, ULBP2/5/6, ULBP3, and ULBP4 on two PDTO lines B2MWT and B2MKO after IFNγ pre-stimulation. e. Flow cytometry gating strategy on γδ T cell samples for analysis of intracellular staining after stimulation with PDTOs in the presence of NKG2D ligand blocking. Lymphocyte population was further gated on single cells, live and CD3+ cells, followed by γδ TCR+ and CD8+ as well as CD8– cells. Reactivity of final population was based on IFNγ+ or CD107a+ cells.

    Journal: bioRxiv

    Article Title: γδ T cells are effectors of immune checkpoint blockade in mismatch repair-deficient colon cancers with antigen presentation defects

    doi: 10.1101/2021.10.14.464229

    Figure Lengend Snippet: a. Flow cytometry gating strategy on PDTO cells for analysis of surface staining. Selected cells were gated on single, live cells before quantification of staining signal. b. Histogram representation and count for surface staining of MHC-I, PD-L1, and β2m expression on two PDTO lines B2MWT and B2MKO after IFNγ pre-stimulation. Staining with isotype antibodies for each fluorochrome (PE, APC and FITC) were included as negative control. c. Flow cytometry gating strategy on γδ T cell samples for analysis of intracellular staining to test antitumor reactivity upon PDTO stimulation. Lymphocyte population was further gated on single cells, live and CD3+ cells, γδ TCR+ cells and CD8+ as well as CD8–CD4– cells. Reactivity of the sample was based on IFNγ+ cells of the selected population. d. Histogram representation and count for surface staining of NKG2D ligands MICA/B, ULBP1, ULBP2/5/6, ULBP3, and ULBP4 on two PDTO lines B2MWT and B2MKO after IFNγ pre-stimulation. e. Flow cytometry gating strategy on γδ T cell samples for analysis of intracellular staining after stimulation with PDTOs in the presence of NKG2D ligand blocking. Lymphocyte population was further gated on single cells, live and CD3+ cells, followed by γδ TCR+ and CD8+ as well as CD8– cells. Reactivity of final population was based on IFNγ+ or CD107a+ cells.

    Article Snippet: Briefly, cells were incubated with human Fc receptor block (BioLegend) and stained with the different cell surface antibodies (1:10 anti-CD112-PE [clone R2.525, BD Biosciences], 1:10 anti-CD155-PE [clone 300907, R&D Systems], 1:50 anti-CD277/BTN3A1-PE [clone BT3.1, Miltenyi], 1:100 anti-HLA-A,B,C-FITC [clone W6/32, eBioscience], 1:20 anti-HLA-E­BV421 [clone 3D12, BioLegend], 1:20 anti-HLA-G-APC [clone 87G, BioLegend], 1:300 anti-MICA/B-PE [clone 6D4, BioLegend], 1:10 anti-ULBP1-PE [clone 170818, R&D Systems], 1:20 anti-ULBP2/5/6-PE [clone 165903, R&D Systems], 1:20 anti-ULBP3-PE [clone 166510, R&D Systems], or 1:20 anti-ULBP4-PE [clone 709116, R&D Systems] for 45 min at 4°C.

    Techniques: Flow Cytometry, Staining, Expressing, Negative Control, Blocking Assay